ULTRAVIOLET-FLUORESCENCE MICROSCOPY OF PAINT CROSS SECTIONS:
JOHN M. MESSINGER
ABSTRACT—Cycloheptaamylose-dansyl chloride complex, a new fluorochrome specific for proteins, has been investigated as a stain to identify artists' paint media. Its selectivity and limitations are compared to those of the following commonly available fluorescent and nonfluorescent stains: lissamine rhodamine B sulfonyl chloride, 5-fluorescein isothiocyanate, amido black, Sudan black, rhodamine B, and 2′,7′-dichlorofluorescein. The methods used to examine the characteristics of these stains were reflected visible light microscopy and ultraviolet-fluorescence microscopy. The known samples of artists' media analyzed with the stains were linseed oil, casein, tempera, glair, gelatin, rabbit skin glue, and acrylic emulsions.
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