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Subject: Course on identifying proteins with ELISA

Course on identifying proteins with ELISA

From: Margaret Sawicki <margaret.sawicki<-a>
Date: Friday, May 16, 2014
Course

Identification of protein-based materials in art objects using the
    Enzyme-linked Immunosorbent Assay (ELISA) technique

Art Gallery of New South Wales,
Sydney, Australia
9-11 September 2014

Registration is now open for participation in the course on
identification of protein-based materials in art objects using the
Enzyme-linked Immunosorbent Assay (ELISA) technique, Art Gallery of
New South Wales, Sydney, Australia, 9-11 September 2014.  The course
is an event of the ICOM-CC Wood, Furniture, and Lacquer Working
Group, complementing, but not a part of the ICOM-CC 17th Triennial
Conference in Melbourne, 15-19 September 2014.  The course is open
to all conservators, conservation scientists, and MA conservation
students, particularly to members of ICOM-CC and AICCM.

The three-day workshop will explore newly developed immunological
procedures for the identification of proteins and gums in works of
art.  By different antibody-based techniques complex mixtures of
several proteins and gums can be distinguished, down to a species
level and can be localized on cross sections.  The participants will
have an opportunity to discuss the challenges of protein analysis in
the relevance to conservation and an interpretation of proteinaceous
binding media in works of art.

The course at the AGNSW will be conducted by Dr Julia Schultz,
conservation scientist at the Stuttgart State Academy of Art and
Design, Stuttgart, Germany (see Julia's short biography below).

The course fee is AU$550 (including GST).

The workshop provides instruction in the following procedures with
the aim of identifying proteins and gums in works of art:

    Visible and fluorescent light microscopic examination of
    chemically-stained cross-sections which can provide visual,
    layer-specific information for proteins

    Precision sample collection and preparation prior ELISA permits
    layer-specific compositional information to be obtained

    Fourier transform infrared spectroscopy (FTIR) analysis for
    general classification of sample composition

    Enzyme-linked Immunosorbent assay (ELISA) to simultaneously
    screen for ovalbumin, casein, collagen and gums, and further
    differentiate into fish collagen, collagen specific to sturgeon
    and gum tragacanth.

    Immunofluorescence microscopy (IFM) on selected cross sections
    for localization of the proteins in the layer stratigraphy

Early applications are encouraged due to a strict limit on the
number of places.  If you are interested in participation in this
course please contact

    Dr Malgorzata Sawicki Coordinator
    ICOM-CC Wood, Furniture, and Lacquer Working Group
    margaret.sawicki<-a t->ag< . >nsw< . >gov< . >au

by 27 June 2014

Brief biography of the tutor:  Julia Schultz was trained as a
conservator, specializing in furniture and wooden objects.  She
received a Diploma from the University of Applied Sciences and Arts
Hildesheim (HAWK), Germany in 2003, with a focus on historical
woodworking tools and microbiological contaminated objects.  After a
postgraduate internship in the Department of Decorative Arts and
Sculpture Conservation at the J.  Paul Getty Museum in California
(2003-2004), she obtained an MA in Conservation from HAWK,
researching antibody-based techniques for the identification of
proteinaceous binding media in works of art (2006).  As part of her
PhD dissertation at the State Academy of Art and Design in
Stuttgart, Germany, she spent 2 years as an Andrew W.  Mellon Fellow
in the Department of Scientific Research at The Metropolitan Museum
of Art in New York working on the application of immunological
techniques to the study of proteins and gums in works of art.
Between 2009 -2013 Julia worked as a research assistant at HAWK,
where she was involved in projects related to microbiological
questions in cultural heritage and where she continues her work on
immunological techniques.  Since January 2014 Julia holds a position
of Conservation Scientist at the Stuttgart State Academy of Art and
Design, Stuttgart, Germany.

Dr Malgorzata Sawicki,
Coordinator, ICOM-CC Wood, Furniture, and Lacquer Working Group


                                  ***
                  Conservation DistList Instance 27:45
                  Distributed: Saturday, May 17, 2014
                       Message Id: cdl-27-45-010
                                  ***
Received on Friday, 16 May, 2014

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